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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARAYCOCHEA, S.; SPERANZA, P.; ALVAREZ-VALIN, F. |
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Afiliación : |
SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Applications in Plant Sciences, 2015, v.3, no.10, Article number 1500022. OPEN ACCESS |
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ISSN : |
2168-0450 |
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DOI : |
10.3732/apps.1500022 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 12 October 2015.
The authors thank Fabián Capdeville for his important support at the beginning of this work. This project received financial support from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. S.G. was the recipient of a Master's degree fellowship awarded by Agencia Nacional de Investigación e Innovación (ANII), Uruguay. F.A.?V. and P.S. are researchers from Sistema Nacional de Investigadores (ANII, Uruguay). |
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Contenido : |
ABSTRACT.
Premise of the study: We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. Methods: A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. Results: From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs. This weedy rice was classified based on 23 polymorphic regions identified by comparison with reference chloroplast genomes. We detected recent and past events of genetic material transfer between the chloroplast and nuclear genomes and estimated their occurrence frequency. Discussion: We obtained a high-quality complete chloroplast genome sequence from low-coverage sequencing data. Intergenome DNA transfer appears to be more frequent than previously thought.
© 2015 Garaycochea et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
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Palabras claves : |
Bioinformatic methods; BIOINFORMÁTICA; Chloroplast genome; Next-generation; Sequencing; Weedy rice. |
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Thesagro : |
ARROZ; BIOLOGÍA; CIENCIA DE INFORMACIÓN; ORYZA SATIVA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5184/1/Garaycochea-S.-2015.-Applications-in-plant-Sciences.pdf
https://bsapubs.onlinelibrary.wiley.com/doi/pdf/10.3732/apps.1500022
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Marc : |
LEADER 02579naa a2200301 a 4500
001 1053875
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
022 $a2168-0450
024 7 $a10.3732/apps.1500022$2DOI
100 1 $aGARAYCOCHEA, S.
245 $aA strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing.$h[electronic resource]
260 $c2015
500 $aArticle history: First published: 12 October 2015. The authors thank Fabián Capdeville for his important support at the beginning of this work. This project received financial support from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. S.G. was the recipient of a Master's degree fellowship awarded by Agencia Nacional de Investigación e Innovación (ANII), Uruguay. F.A.?V. and P.S. are researchers from Sistema Nacional de Investigadores (ANII, Uruguay).
520 $aABSTRACT. Premise of the study: We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. Methods: A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. Results: From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs. This weedy rice was classified based on 23 polymorphic regions identified by comparison with reference chloroplast genomes. We detected recent and past events of genetic material transfer between the chloroplast and nuclear genomes and estimated their occurrence frequency. Discussion: We obtained a high-quality complete chloroplast genome sequence from low-coverage sequencing data. Intergenome DNA transfer appears to be more frequent than previously thought. © 2015 Garaycochea et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
650 $aARROZ
650 $aBIOLOGÍA
650 $aCIENCIA DE INFORMACIÓN
650 $aORYZA SATIVA
653 $aBioinformatic methods
653 $aBIOINFORMÁTICA
653 $aChloroplast genome
653 $aNext-generation
653 $aSequencing
653 $aWeedy rice
700 1 $aSPERANZA, P.
700 1 $aALVAREZ-VALIN, F.
773 $tApplications in Plant Sciences, 2015$gv.3, no.10, Article number 1500022. OPEN ACCESS
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
22/04/2021 |
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Actualizado : |
22/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GIORELLO, D.; SBRISSIA, A.F.; DA SILVA, S.C. |
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Afiliación : |
DIEGO GERMAN GIORELLO LEITES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉ FISCHER SBRISSIA, Universidade do Estado de Santa Catarina, Lages (SC), Brazil; SILA CARNEIRO DA SILVA, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Zootecnia, Piracicaba (SP), Brazil. |
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Título : |
Herbage accumulation, morphological composition, and nutritive value of Paspalum notatum CV. INIA Sepé [Acumulación de forraje, composición morfológica y valor nutritivo de
Paspalum notatum CV. INIA Sepé]. [Acúmulo de forragem, composição morfológica e valor nutritivo de Paspalum notatum CV. INIA Sepé]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2021, 25(1):1-14. Doi: https: https://doi.org/10.31285/AGRO.25.348 |
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ISSN : |
e-ISSN: 2301-1548 |
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DOI : |
10.31285/AGRO.25.348 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 2 Apr 2020; Accepted 10 Dec 2020; Published 20 Apr 2021. Acknowledgments: The experimental study was funded by INIA (Na#2;tional Institute for Agricultural Research), while the Doctoral training was funded by a grant awarded by ANII (National Agency for Research and Innova#2;tion).
Editor: Rafael Reyno, Instituto Nacional de Investigación Agropecuaria (INIA), Tacuarembó, Uruguay. |
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Contenido : |
Abstract: Defoliation strategies determine sward canopy structure and affect forage accumulation and nutritive value. The objective of this study was to determine forage accumulation, morphological composition and nutritive value of INIA Sepé subjected to combinations of three frequencies (defoliation at 90%, 95% and maximum canopy light interception during regrowth ?LI90%, LI95% and LIMax), and two severities of defoliation (40 and 60% of the precutting height). The experiment was carried out from November 15th, 2018 to March 31st, 2019 in Tacuarembó. Measurements of LI and canopy height, forage mass morphological composition and nutritional value were carried out. Forage mass was greater in Feb-March. The percentage of leaves was greater for the higher defoliation frequency treatment (LI90%). The percentage of stems did not vary among treatments (average of 23%). The percentage of dead material was greater for the lower defoliation frequency treatment (LIMax) and 40% defoliation severity. Greater ADF and NDF values and smaller digestibility were recorded for LIMax/40% combination. For LI90%, greater ADF and NDF, and smaller digestibility were recorded for the 60% defoliation severity (Feb-March). Forage accumulation was greater for LI90% and LI95%. INIA Sepé shows large flexibility to defoliation management, and the optimal pre-cutting target should not exceed LI95% (i.e. 30 cm of canopy height) in order to ensure production of high nutritive value forage and harvest efficiency. Resumen: El manejo de la defoliación es determinante de la estructura del dosel forrajero y afecta la acumulación de forraje y su valor nutricional. El objetivo de este trabajo fue determinar la acumulación de forraje, su composición morfológica y el valor nutritivo de INIA Sepé sometido a la combinación de tres frecuencias (defoliación a 90 %, 95 % y máxima interceptación de luz por el dosel forrajero durante el rebrote —IL90%, IL95% e ILMÁX) y dos severi#2;dades de defoliación (40 y 60 % de la altura precorte). El experimento fue realizado desde el 15 de noviembre de 2018 hasta el 31 de marzo de 2019 en Tacuarembó. Fueron realizadas evaluaciones de IL y altura del dosel forrajero, masa de forraje, composición morfológica y valor nutritivo del forraje. La masa de forraje fue mayor en Feb-Mar. El porcentaje de hojas fue mayor para la mayor frecuencia de defoliación (IL90%). El porcentaje de tallos no varió entre tratamientos (promedio de 23 %). El porcentaje de material muerto fue mayor para la menor frecuencia (ILmáx) y para la severidad 40 %. Mayores valores de FDA, FDN y menor digestibilidad fueron registra#2;dos para la combinación ILmáx/40 %. Para IL90% mayor FDA y FDN y menor digestibilidad fueron registrados para la severidad 60 % (Feb-Mar). La acumulación de forraje fue mayor para IL90% e IL95%. INIA Sepé posee gran flexibilidad de manejo, mientras que la meta precorte óptima no debe exceder 95 % de IL (30 cm de altura), como forma de asegurar producción de forraje con elevado valor nutritivo y elevada eficiencia de cosecha. Resumo: O manejo da desfolhação é determinante da estrutura do dossel forrageiro e afeta a produção e o valor nutritivo da forragem. O objetivo deste trabalho foi determinar o acúmulo de forragem, a composição morfológica e o valor nutritivo de INIA Sepé submetido à combinação de três frequências (desfolhação aos 90%, 95% e máxima
interceptação de luz pelo dossel forrageiro durante a rebrotação – IL90%, IL95% e ILMáx) e duas severidades de desfolhação (40 e 60% da altura pré-corte). O experimento foi realizado de 15 de novembro de 2018 a 31 de março de 2019, em Tacuarembó. Foram realizadas avaliações de IL e altura do dossel forrageiro, massa de forragem, composição morfológica e valor nutritivo da forragem. A massa de forragem foi maior em Fev-Mar. A porcentagem de folhas foi maior para a maior frequência de desfolhação (IL90%). A porcentagem de colmos não variou entre tratamentos (média de 23%). Por outro lado, a porcentagem de material morto foi maior para a menor frequência (ILMáx) e para a severidade 40%. Maiores valores de FDA e FDN assim como menores de digestibilidade foram registrados para a combinação ILMáx/40%. Para IL90% maior FDA, FDN e menor diges#2;tibilidade foram registrados para a severidade 60% (Fev-Mar). O acúmulo de forragem foi maior para IL90% e IL95%. INIA Sepé possui grande flexibilidade de manejo, sendo a meta pré-corte ótima não deve exceder 95% de IL (30 cm de altura) como forma de assegurar produção de forragem com elevado valor nutritivo e elevada eficiência de colheita. MenosAbstract: Defoliation strategies determine sward canopy structure and affect forage accumulation and nutritive value. The objective of this study was to determine forage accumulation, morphological composition and nutritive value of INIA Sepé subjected to combinations of three frequencies (defoliation at 90%, 95% and maximum canopy light interception during regrowth ?LI90%, LI95% and LIMax), and two severities of defoliation (40 and 60% of the precutting height). The experiment was carried out from November 15th, 2018 to March 31st, 2019 in Tacuarembó. Measurements of LI and canopy height, forage mass morphological composition and nutritional value were carried out. Forage mass was greater in Feb-March. The percentage of leaves was greater for the higher defoliation frequency treatment (LI90%). The percentage of stems did not vary among treatments (average of 23%). The percentage of dead material was greater for the lower defoliation frequency treatment (LIMax) and 40% defoliation severity. Greater ADF and NDF values and smaller digestibility were recorded for LIMax/40% combination. For LI90%, greater ADF and NDF, and smaller digestibility were recorded for the 60% defoliation severity (Feb-March). Forage accumulation was greater for LI90% and LI95%. INIA Sepé shows large flexibility to defoliation management, and the optimal pre-cutting target should not exceed LI95% (i.e. 30 cm of canopy height) in order to ensure production of high nutritive value forage and harvest effic... Presentar Todo |
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Palabras claves : |
ACUMULACIÓN DE FORRAJE; HERBAGE ACCUMULATION; NUTRITIONAL VALUE; PASPALUM NOTATUM; VALOR NUTRITIVO. |
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Thesagro : |
PASTURAS. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15508/1/Giorello-Agrociencia2021.pdf
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Marc : |
LEADER 06087naa a2200253 a 4500
001 1061989
005 2021-04-22
008 2021 bl uuuu u00u1 u #d
022 $ae-ISSN: 2301-1548
024 7 $a10.31285/AGRO.25.348$2DOI
100 1 $aGIORELLO, D.
245 $aHerbage accumulation, morphological composition, and nutritive value of Paspalum notatum CV. INIA Sepé [Acumulación de forraje, composición morfológica y valor nutritivo de Paspalum notatum CV. INIA Sepé]. [Acúmulo de forragem, composição morfológica e valor nutritivo de Paspalum notatum CV. INIA Sepé].$h[electronic resource]
260 $c2021
500 $aArticle history: Received 2 Apr 2020; Accepted 10 Dec 2020; Published 20 Apr 2021. Acknowledgments: The experimental study was funded by INIA (Na#2;tional Institute for Agricultural Research), while the Doctoral training was funded by a grant awarded by ANII (National Agency for Research and Innova#2;tion). Editor: Rafael Reyno, Instituto Nacional de Investigación Agropecuaria (INIA), Tacuarembó, Uruguay.
520 $aAbstract: Defoliation strategies determine sward canopy structure and affect forage accumulation and nutritive value. The objective of this study was to determine forage accumulation, morphological composition and nutritive value of INIA Sepé subjected to combinations of three frequencies (defoliation at 90%, 95% and maximum canopy light interception during regrowth ?LI90%, LI95% and LIMax), and two severities of defoliation (40 and 60% of the precutting height). The experiment was carried out from November 15th, 2018 to March 31st, 2019 in Tacuarembó. Measurements of LI and canopy height, forage mass morphological composition and nutritional value were carried out. Forage mass was greater in Feb-March. The percentage of leaves was greater for the higher defoliation frequency treatment (LI90%). The percentage of stems did not vary among treatments (average of 23%). The percentage of dead material was greater for the lower defoliation frequency treatment (LIMax) and 40% defoliation severity. Greater ADF and NDF values and smaller digestibility were recorded for LIMax/40% combination. For LI90%, greater ADF and NDF, and smaller digestibility were recorded for the 60% defoliation severity (Feb-March). Forage accumulation was greater for LI90% and LI95%. INIA Sepé shows large flexibility to defoliation management, and the optimal pre-cutting target should not exceed LI95% (i.e. 30 cm of canopy height) in order to ensure production of high nutritive value forage and harvest efficiency. Resumen: El manejo de la defoliación es determinante de la estructura del dosel forrajero y afecta la acumulación de forraje y su valor nutricional. El objetivo de este trabajo fue determinar la acumulación de forraje, su composición morfológica y el valor nutritivo de INIA Sepé sometido a la combinación de tres frecuencias (defoliación a 90 %, 95 % y máxima interceptación de luz por el dosel forrajero durante el rebrote —IL90%, IL95% e ILMÁX) y dos severi#2;dades de defoliación (40 y 60 % de la altura precorte). El experimento fue realizado desde el 15 de noviembre de 2018 hasta el 31 de marzo de 2019 en Tacuarembó. Fueron realizadas evaluaciones de IL y altura del dosel forrajero, masa de forraje, composición morfológica y valor nutritivo del forraje. La masa de forraje fue mayor en Feb-Mar. El porcentaje de hojas fue mayor para la mayor frecuencia de defoliación (IL90%). El porcentaje de tallos no varió entre tratamientos (promedio de 23 %). El porcentaje de material muerto fue mayor para la menor frecuencia (ILmáx) y para la severidad 40 %. Mayores valores de FDA, FDN y menor digestibilidad fueron registra#2;dos para la combinación ILmáx/40 %. Para IL90% mayor FDA y FDN y menor digestibilidad fueron registrados para la severidad 60 % (Feb-Mar). La acumulación de forraje fue mayor para IL90% e IL95%. INIA Sepé posee gran flexibilidad de manejo, mientras que la meta precorte óptima no debe exceder 95 % de IL (30 cm de altura), como forma de asegurar producción de forraje con elevado valor nutritivo y elevada eficiencia de cosecha. Resumo: O manejo da desfolhação é determinante da estrutura do dossel forrageiro e afeta a produção e o valor nutritivo da forragem. O objetivo deste trabalho foi determinar o acúmulo de forragem, a composição morfológica e o valor nutritivo de INIA Sepé submetido à combinação de três frequências (desfolhação aos 90%, 95% e máxima interceptação de luz pelo dossel forrageiro durante a rebrotação – IL90%, IL95% e ILMáx) e duas severidades de desfolhação (40 e 60% da altura pré-corte). O experimento foi realizado de 15 de novembro de 2018 a 31 de março de 2019, em Tacuarembó. Foram realizadas avaliações de IL e altura do dossel forrageiro, massa de forragem, composição morfológica e valor nutritivo da forragem. A massa de forragem foi maior em Fev-Mar. A porcentagem de folhas foi maior para a maior frequência de desfolhação (IL90%). A porcentagem de colmos não variou entre tratamentos (média de 23%). Por outro lado, a porcentagem de material morto foi maior para a menor frequência (ILMáx) e para a severidade 40%. Maiores valores de FDA e FDN assim como menores de digestibilidade foram registrados para a combinação ILMáx/40%. Para IL90% maior FDA, FDN e menor diges#2;tibilidade foram registrados para a severidade 60% (Fev-Mar). O acúmulo de forragem foi maior para IL90% e IL95%. INIA Sepé possui grande flexibilidade de manejo, sendo a meta pré-corte ótima não deve exceder 95% de IL (30 cm de altura) como forma de assegurar produção de forragem com elevado valor nutritivo e elevada eficiência de colheita.
650 $aPASTURAS
653 $aACUMULACIÓN DE FORRAJE
653 $aHERBAGE ACCUMULATION
653 $aNUTRITIONAL VALUE
653 $aPASPALUM NOTATUM
653 $aVALOR NUTRITIVO
700 1 $aSBRISSIA, A.F.
700 1 $aDA SILVA, S.C.
773 $tAgrociencia Uruguay, 2021, 25(1):1-14. Doi: https: https://doi.org/10.31285/AGRO.25.348
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